rna synthesis inhibitor actinomycin d actd Search Results


actinomycin d  (Oncoscience ag)
90
Oncoscience ag actinomycin d
Actinomycin D, supplied by Oncoscience ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant proteins actinomycin d actd thermo fisher scientific  (Thermo Fisher)
97
Thermo Fisher recombinant proteins actinomycin d actd thermo fisher scientific
Figure 5. FUS, but Not TDP-43, HuR, SF1, or TIA-1, Shuttles from the Nucleus to the Cytoplasm after PARP-1 Activation in H2O2-Treated Cells (A) The relative distribution of PAR and five other RNA-binding proteins—SF1, TDP-43, TIA-1, FUS, and HuR—between the cytoplasm and nucleus of HeLa cells was measured at different times after H2O2 treatment (300 mM). The integrated fluorescence intensities were measured at the single-cell level by using cell profiler software and secondary antibody fluorescence. Statistical analysis of protein translocation was performed under the indicated conditions. Horizontal bars indicate mean; n > 244. Note that FUS translocation takes place after 15 min, which is not observed for TDP-43. (B) Upper left panel: anti-PAR fluorescence after H2O2 treatment (300 mM) for 10 min in the presence or absence of olabarib (10 mM). Upper right panels: sub- cellular FUS distribution after the indicated treatments. Lower panels: statistical analysis of FUS translocation under indicated conditions. H2O2 treatment: 300 mM, 90 min. Horizontal bars indicate mean. **p < 0.05, paired t test; n.s., non-significant; n > 278. Olaparib treatment or silencing PARP-1 inhibits the shuttling of FUS from the nucleus to the cytoplasm. (C) Subcellular distribution of TDP-43 in HeLa cells under the same conditions as in (B). Olaparib does not impair the nucleocytoplasmic shuttling of TDP-43. (D) Left panels: subcellular distribution of HuR in HeLa cells under the indicated conditions. Right panel: analysis of the nucleocytoplasmic shuttling of HuR. **p < 0.05, paired t test; n = 240. Actinomycin D, <t>ActD:</t> 5 mg/mL. Scale bar, 10 mm. H2O2 treatment prevents the translocation of HuR in response to ActD in HeLa cells in a PAR-dependent manner. n.s., not significant.
Recombinant Proteins Actinomycin D Actd Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna synthesis inhibitor actinomycin d  (Selleck Chemicals)
96
Selleck Chemicals rna synthesis inhibitor actinomycin d
Figure 5. FUS, but Not TDP-43, HuR, SF1, or TIA-1, Shuttles from the Nucleus to the Cytoplasm after PARP-1 Activation in H2O2-Treated Cells (A) The relative distribution of PAR and five other RNA-binding proteins—SF1, TDP-43, TIA-1, FUS, and HuR—between the cytoplasm and nucleus of HeLa cells was measured at different times after H2O2 treatment (300 mM). The integrated fluorescence intensities were measured at the single-cell level by using cell profiler software and secondary antibody fluorescence. Statistical analysis of protein translocation was performed under the indicated conditions. Horizontal bars indicate mean; n > 244. Note that FUS translocation takes place after 15 min, which is not observed for TDP-43. (B) Upper left panel: anti-PAR fluorescence after H2O2 treatment (300 mM) for 10 min in the presence or absence of olabarib (10 mM). Upper right panels: sub- cellular FUS distribution after the indicated treatments. Lower panels: statistical analysis of FUS translocation under indicated conditions. H2O2 treatment: 300 mM, 90 min. Horizontal bars indicate mean. **p < 0.05, paired t test; n.s., non-significant; n > 278. Olaparib treatment or silencing PARP-1 inhibits the shuttling of FUS from the nucleus to the cytoplasm. (C) Subcellular distribution of TDP-43 in HeLa cells under the same conditions as in (B). Olaparib does not impair the nucleocytoplasmic shuttling of TDP-43. (D) Left panels: subcellular distribution of HuR in HeLa cells under the indicated conditions. Right panel: analysis of the nucleocytoplasmic shuttling of HuR. **p < 0.05, paired t test; n = 240. Actinomycin D, <t>ActD:</t> 5 mg/mL. Scale bar, 10 mm. H2O2 treatment prevents the translocation of HuR in response to ActD in HeLa cells in a PAR-dependent manner. n.s., not significant.
Rna Synthesis Inhibitor Actinomycin D, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mrna transcription inhibitor actinomycin d  (MedChemExpress)
98
MedChemExpress mrna transcription inhibitor actinomycin d
Figure 5. FUS, but Not TDP-43, HuR, SF1, or TIA-1, Shuttles from the Nucleus to the Cytoplasm after PARP-1 Activation in H2O2-Treated Cells (A) The relative distribution of PAR and five other RNA-binding proteins—SF1, TDP-43, TIA-1, FUS, and HuR—between the cytoplasm and nucleus of HeLa cells was measured at different times after H2O2 treatment (300 mM). The integrated fluorescence intensities were measured at the single-cell level by using cell profiler software and secondary antibody fluorescence. Statistical analysis of protein translocation was performed under the indicated conditions. Horizontal bars indicate mean; n > 244. Note that FUS translocation takes place after 15 min, which is not observed for TDP-43. (B) Upper left panel: anti-PAR fluorescence after H2O2 treatment (300 mM) for 10 min in the presence or absence of olabarib (10 mM). Upper right panels: sub- cellular FUS distribution after the indicated treatments. Lower panels: statistical analysis of FUS translocation under indicated conditions. H2O2 treatment: 300 mM, 90 min. Horizontal bars indicate mean. **p < 0.05, paired t test; n.s., non-significant; n > 278. Olaparib treatment or silencing PARP-1 inhibits the shuttling of FUS from the nucleus to the cytoplasm. (C) Subcellular distribution of TDP-43 in HeLa cells under the same conditions as in (B). Olaparib does not impair the nucleocytoplasmic shuttling of TDP-43. (D) Left panels: subcellular distribution of HuR in HeLa cells under the indicated conditions. Right panel: analysis of the nucleocytoplasmic shuttling of HuR. **p < 0.05, paired t test; n = 240. Actinomycin D, <t>ActD:</t> 5 mg/mL. Scale bar, 10 mm. H2O2 treatment prevents the translocation of HuR in response to ActD in HeLa cells in a PAR-dependent manner. n.s., not significant.
Mrna Transcription Inhibitor Actinomycin D, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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actinomycin d  (Cell Signaling Technology Inc)
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Figure 5. FUS, but Not TDP-43, HuR, SF1, or TIA-1, Shuttles from the Nucleus to the Cytoplasm after PARP-1 Activation in H2O2-Treated Cells (A) The relative distribution of PAR and five other RNA-binding proteins—SF1, TDP-43, TIA-1, FUS, and HuR—between the cytoplasm and nucleus of HeLa cells was measured at different times after H2O2 treatment (300 mM). The integrated fluorescence intensities were measured at the single-cell level by using cell profiler software and secondary antibody fluorescence. Statistical analysis of protein translocation was performed under the indicated conditions. Horizontal bars indicate mean; n > 244. Note that FUS translocation takes place after 15 min, which is not observed for TDP-43. (B) Upper left panel: anti-PAR fluorescence after H2O2 treatment (300 mM) for 10 min in the presence or absence of olabarib (10 mM). Upper right panels: sub- cellular FUS distribution after the indicated treatments. Lower panels: statistical analysis of FUS translocation under indicated conditions. H2O2 treatment: 300 mM, 90 min. Horizontal bars indicate mean. **p < 0.05, paired t test; n.s., non-significant; n > 278. Olaparib treatment or silencing PARP-1 inhibits the shuttling of FUS from the nucleus to the cytoplasm. (C) Subcellular distribution of TDP-43 in HeLa cells under the same conditions as in (B). Olaparib does not impair the nucleocytoplasmic shuttling of TDP-43. (D) Left panels: subcellular distribution of HuR in HeLa cells under the indicated conditions. Right panel: analysis of the nucleocytoplasmic shuttling of HuR. **p < 0.05, paired t test; n = 240. Actinomycin D, <t>ActD:</t> 5 mg/mL. Scale bar, 10 mm. H2O2 treatment prevents the translocation of HuR in response to ActD in HeLa cells in a PAR-dependent manner. n.s., not significant.
Actinomycin D, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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actinomycin-d act-d  (ICN Biomedicals)
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Figure 5. FUS, but Not TDP-43, HuR, SF1, or TIA-1, Shuttles from the Nucleus to the Cytoplasm after PARP-1 Activation in H2O2-Treated Cells (A) The relative distribution of PAR and five other RNA-binding proteins—SF1, TDP-43, TIA-1, FUS, and HuR—between the cytoplasm and nucleus of HeLa cells was measured at different times after H2O2 treatment (300 mM). The integrated fluorescence intensities were measured at the single-cell level by using cell profiler software and secondary antibody fluorescence. Statistical analysis of protein translocation was performed under the indicated conditions. Horizontal bars indicate mean; n > 244. Note that FUS translocation takes place after 15 min, which is not observed for TDP-43. (B) Upper left panel: anti-PAR fluorescence after H2O2 treatment (300 mM) for 10 min in the presence or absence of olabarib (10 mM). Upper right panels: sub- cellular FUS distribution after the indicated treatments. Lower panels: statistical analysis of FUS translocation under indicated conditions. H2O2 treatment: 300 mM, 90 min. Horizontal bars indicate mean. **p < 0.05, paired t test; n.s., non-significant; n > 278. Olaparib treatment or silencing PARP-1 inhibits the shuttling of FUS from the nucleus to the cytoplasm. (C) Subcellular distribution of TDP-43 in HeLa cells under the same conditions as in (B). Olaparib does not impair the nucleocytoplasmic shuttling of TDP-43. (D) Left panels: subcellular distribution of HuR in HeLa cells under the indicated conditions. Right panel: analysis of the nucleocytoplasmic shuttling of HuR. **p < 0.05, paired t test; n = 240. Actinomycin D, <t>ActD:</t> 5 mg/mL. Scale bar, 10 mm. H2O2 treatment prevents the translocation of HuR in response to ActD in HeLa cells in a PAR-dependent manner. n.s., not significant.
Actinomycin D Act D, supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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actinomycin d act d  (ApexBio)
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Figure 5. FUS, but Not TDP-43, HuR, SF1, or TIA-1, Shuttles from the Nucleus to the Cytoplasm after PARP-1 Activation in H2O2-Treated Cells (A) The relative distribution of PAR and five other RNA-binding proteins—SF1, TDP-43, TIA-1, FUS, and HuR—between the cytoplasm and nucleus of HeLa cells was measured at different times after H2O2 treatment (300 mM). The integrated fluorescence intensities were measured at the single-cell level by using cell profiler software and secondary antibody fluorescence. Statistical analysis of protein translocation was performed under the indicated conditions. Horizontal bars indicate mean; n > 244. Note that FUS translocation takes place after 15 min, which is not observed for TDP-43. (B) Upper left panel: anti-PAR fluorescence after H2O2 treatment (300 mM) for 10 min in the presence or absence of olabarib (10 mM). Upper right panels: sub- cellular FUS distribution after the indicated treatments. Lower panels: statistical analysis of FUS translocation under indicated conditions. H2O2 treatment: 300 mM, 90 min. Horizontal bars indicate mean. **p < 0.05, paired t test; n.s., non-significant; n > 278. Olaparib treatment or silencing PARP-1 inhibits the shuttling of FUS from the nucleus to the cytoplasm. (C) Subcellular distribution of TDP-43 in HeLa cells under the same conditions as in (B). Olaparib does not impair the nucleocytoplasmic shuttling of TDP-43. (D) Left panels: subcellular distribution of HuR in HeLa cells under the indicated conditions. Right panel: analysis of the nucleocytoplasmic shuttling of HuR. **p < 0.05, paired t test; n = 240. Actinomycin D, <t>ActD:</t> 5 mg/mL. Scale bar, 10 mm. H2O2 treatment prevents the translocation of HuR in response to ActD in HeLa cells in a PAR-dependent manner. n.s., not significant.
Actinomycin D Act D, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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actinomycin d  (GlpBio Technology Inc)
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Figure 5. FUS, but Not TDP-43, HuR, SF1, or TIA-1, Shuttles from the Nucleus to the Cytoplasm after PARP-1 Activation in H2O2-Treated Cells (A) The relative distribution of PAR and five other RNA-binding proteins—SF1, TDP-43, TIA-1, FUS, and HuR—between the cytoplasm and nucleus of HeLa cells was measured at different times after H2O2 treatment (300 mM). The integrated fluorescence intensities were measured at the single-cell level by using cell profiler software and secondary antibody fluorescence. Statistical analysis of protein translocation was performed under the indicated conditions. Horizontal bars indicate mean; n > 244. Note that FUS translocation takes place after 15 min, which is not observed for TDP-43. (B) Upper left panel: anti-PAR fluorescence after H2O2 treatment (300 mM) for 10 min in the presence or absence of olabarib (10 mM). Upper right panels: sub- cellular FUS distribution after the indicated treatments. Lower panels: statistical analysis of FUS translocation under indicated conditions. H2O2 treatment: 300 mM, 90 min. Horizontal bars indicate mean. **p < 0.05, paired t test; n.s., non-significant; n > 278. Olaparib treatment or silencing PARP-1 inhibits the shuttling of FUS from the nucleus to the cytoplasm. (C) Subcellular distribution of TDP-43 in HeLa cells under the same conditions as in (B). Olaparib does not impair the nucleocytoplasmic shuttling of TDP-43. (D) Left panels: subcellular distribution of HuR in HeLa cells under the indicated conditions. Right panel: analysis of the nucleocytoplasmic shuttling of HuR. **p < 0.05, paired t test; n = 240. Actinomycin D, <t>ActD:</t> 5 mg/mL. Scale bar, 10 mm. H2O2 treatment prevents the translocation of HuR in response to ActD in HeLa cells in a PAR-dependent manner. n.s., not significant.
Actinomycin D, supplied by GlpBio Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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actinomycin d (act.d, glpbio)  (GlpBio Technology Inc)
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Figure 5. FUS, but Not TDP-43, HuR, SF1, or TIA-1, Shuttles from the Nucleus to the Cytoplasm after PARP-1 Activation in H2O2-Treated Cells (A) The relative distribution of PAR and five other RNA-binding proteins—SF1, TDP-43, TIA-1, FUS, and HuR—between the cytoplasm and nucleus of HeLa cells was measured at different times after H2O2 treatment (300 mM). The integrated fluorescence intensities were measured at the single-cell level by using cell profiler software and secondary antibody fluorescence. Statistical analysis of protein translocation was performed under the indicated conditions. Horizontal bars indicate mean; n > 244. Note that FUS translocation takes place after 15 min, which is not observed for TDP-43. (B) Upper left panel: anti-PAR fluorescence after H2O2 treatment (300 mM) for 10 min in the presence or absence of olabarib (10 mM). Upper right panels: sub- cellular FUS distribution after the indicated treatments. Lower panels: statistical analysis of FUS translocation under indicated conditions. H2O2 treatment: 300 mM, 90 min. Horizontal bars indicate mean. **p < 0.05, paired t test; n.s., non-significant; n > 278. Olaparib treatment or silencing PARP-1 inhibits the shuttling of FUS from the nucleus to the cytoplasm. (C) Subcellular distribution of TDP-43 in HeLa cells under the same conditions as in (B). Olaparib does not impair the nucleocytoplasmic shuttling of TDP-43. (D) Left panels: subcellular distribution of HuR in HeLa cells under the indicated conditions. Right panel: analysis of the nucleocytoplasmic shuttling of HuR. **p < 0.05, paired t test; n = 240. Actinomycin D, <t>ActD:</t> 5 mg/mL. Scale bar, 10 mm. H2O2 treatment prevents the translocation of HuR in response to ActD in HeLa cells in a PAR-dependent manner. n.s., not significant.
Actinomycin D (Act.D, Glpbio), supplied by GlpBio Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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actinomycin d (actd)  (ApexBio)
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ApexBio actinomycin d (actd)
Figure 5. FUS, but Not TDP-43, HuR, SF1, or TIA-1, Shuttles from the Nucleus to the Cytoplasm after PARP-1 Activation in H2O2-Treated Cells (A) The relative distribution of PAR and five other RNA-binding proteins—SF1, TDP-43, TIA-1, FUS, and HuR—between the cytoplasm and nucleus of HeLa cells was measured at different times after H2O2 treatment (300 mM). The integrated fluorescence intensities were measured at the single-cell level by using cell profiler software and secondary antibody fluorescence. Statistical analysis of protein translocation was performed under the indicated conditions. Horizontal bars indicate mean; n > 244. Note that FUS translocation takes place after 15 min, which is not observed for TDP-43. (B) Upper left panel: anti-PAR fluorescence after H2O2 treatment (300 mM) for 10 min in the presence or absence of olabarib (10 mM). Upper right panels: sub- cellular FUS distribution after the indicated treatments. Lower panels: statistical analysis of FUS translocation under indicated conditions. H2O2 treatment: 300 mM, 90 min. Horizontal bars indicate mean. **p < 0.05, paired t test; n.s., non-significant; n > 278. Olaparib treatment or silencing PARP-1 inhibits the shuttling of FUS from the nucleus to the cytoplasm. (C) Subcellular distribution of TDP-43 in HeLa cells under the same conditions as in (B). Olaparib does not impair the nucleocytoplasmic shuttling of TDP-43. (D) Left panels: subcellular distribution of HuR in HeLa cells under the indicated conditions. Right panel: analysis of the nucleocytoplasmic shuttling of HuR. **p < 0.05, paired t test; n = 240. Actinomycin D, <t>ActD:</t> 5 mg/mL. Scale bar, 10 mm. H2O2 treatment prevents the translocation of HuR in response to ActD in HeLa cells in a PAR-dependent manner. n.s., not significant.
Actinomycin D (Actd), supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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actinomycin d  (Abbott Laboratories)
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Figure 5. FUS, but Not TDP-43, HuR, SF1, or TIA-1, Shuttles from the Nucleus to the Cytoplasm after PARP-1 Activation in H2O2-Treated Cells (A) The relative distribution of PAR and five other RNA-binding proteins—SF1, TDP-43, TIA-1, FUS, and HuR—between the cytoplasm and nucleus of HeLa cells was measured at different times after H2O2 treatment (300 mM). The integrated fluorescence intensities were measured at the single-cell level by using cell profiler software and secondary antibody fluorescence. Statistical analysis of protein translocation was performed under the indicated conditions. Horizontal bars indicate mean; n > 244. Note that FUS translocation takes place after 15 min, which is not observed for TDP-43. (B) Upper left panel: anti-PAR fluorescence after H2O2 treatment (300 mM) for 10 min in the presence or absence of olabarib (10 mM). Upper right panels: sub- cellular FUS distribution after the indicated treatments. Lower panels: statistical analysis of FUS translocation under indicated conditions. H2O2 treatment: 300 mM, 90 min. Horizontal bars indicate mean. **p < 0.05, paired t test; n.s., non-significant; n > 278. Olaparib treatment or silencing PARP-1 inhibits the shuttling of FUS from the nucleus to the cytoplasm. (C) Subcellular distribution of TDP-43 in HeLa cells under the same conditions as in (B). Olaparib does not impair the nucleocytoplasmic shuttling of TDP-43. (D) Left panels: subcellular distribution of HuR in HeLa cells under the indicated conditions. Right panel: analysis of the nucleocytoplasmic shuttling of HuR. **p < 0.05, paired t test; n = 240. Actinomycin D, <t>ActD:</t> 5 mg/mL. Scale bar, 10 mm. H2O2 treatment prevents the translocation of HuR in response to ActD in HeLa cells in a PAR-dependent manner. n.s., not significant.
Actinomycin D, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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actinomycin d (act-d)  (Adooq Bioscience LLC)
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Figure 5. FUS, but Not TDP-43, HuR, SF1, or TIA-1, Shuttles from the Nucleus to the Cytoplasm after PARP-1 Activation in H2O2-Treated Cells (A) The relative distribution of PAR and five other RNA-binding proteins—SF1, TDP-43, TIA-1, FUS, and HuR—between the cytoplasm and nucleus of HeLa cells was measured at different times after H2O2 treatment (300 mM). The integrated fluorescence intensities were measured at the single-cell level by using cell profiler software and secondary antibody fluorescence. Statistical analysis of protein translocation was performed under the indicated conditions. Horizontal bars indicate mean; n > 244. Note that FUS translocation takes place after 15 min, which is not observed for TDP-43. (B) Upper left panel: anti-PAR fluorescence after H2O2 treatment (300 mM) for 10 min in the presence or absence of olabarib (10 mM). Upper right panels: sub- cellular FUS distribution after the indicated treatments. Lower panels: statistical analysis of FUS translocation under indicated conditions. H2O2 treatment: 300 mM, 90 min. Horizontal bars indicate mean. **p < 0.05, paired t test; n.s., non-significant; n > 278. Olaparib treatment or silencing PARP-1 inhibits the shuttling of FUS from the nucleus to the cytoplasm. (C) Subcellular distribution of TDP-43 in HeLa cells under the same conditions as in (B). Olaparib does not impair the nucleocytoplasmic shuttling of TDP-43. (D) Left panels: subcellular distribution of HuR in HeLa cells under the indicated conditions. Right panel: analysis of the nucleocytoplasmic shuttling of HuR. **p < 0.05, paired t test; n = 240. Actinomycin D, <t>ActD:</t> 5 mg/mL. Scale bar, 10 mm. H2O2 treatment prevents the translocation of HuR in response to ActD in HeLa cells in a PAR-dependent manner. n.s., not significant.
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Image Search Results


Figure 5. FUS, but Not TDP-43, HuR, SF1, or TIA-1, Shuttles from the Nucleus to the Cytoplasm after PARP-1 Activation in H2O2-Treated Cells (A) The relative distribution of PAR and five other RNA-binding proteins—SF1, TDP-43, TIA-1, FUS, and HuR—between the cytoplasm and nucleus of HeLa cells was measured at different times after H2O2 treatment (300 mM). The integrated fluorescence intensities were measured at the single-cell level by using cell profiler software and secondary antibody fluorescence. Statistical analysis of protein translocation was performed under the indicated conditions. Horizontal bars indicate mean; n > 244. Note that FUS translocation takes place after 15 min, which is not observed for TDP-43. (B) Upper left panel: anti-PAR fluorescence after H2O2 treatment (300 mM) for 10 min in the presence or absence of olabarib (10 mM). Upper right panels: sub- cellular FUS distribution after the indicated treatments. Lower panels: statistical analysis of FUS translocation under indicated conditions. H2O2 treatment: 300 mM, 90 min. Horizontal bars indicate mean. **p < 0.05, paired t test; n.s., non-significant; n > 278. Olaparib treatment or silencing PARP-1 inhibits the shuttling of FUS from the nucleus to the cytoplasm. (C) Subcellular distribution of TDP-43 in HeLa cells under the same conditions as in (B). Olaparib does not impair the nucleocytoplasmic shuttling of TDP-43. (D) Left panels: subcellular distribution of HuR in HeLa cells under the indicated conditions. Right panel: analysis of the nucleocytoplasmic shuttling of HuR. **p < 0.05, paired t test; n = 240. Actinomycin D, ActD: 5 mg/mL. Scale bar, 10 mm. H2O2 treatment prevents the translocation of HuR in response to ActD in HeLa cells in a PAR-dependent manner. n.s., not significant.

Journal: Cell reports

Article Title: PARP-1 Activation Directs FUS to DNA Damage Sites to Form PARG-Reversible Compartments Enriched in Damaged DNA.

doi: 10.1016/j.celrep.2019.04.031

Figure Lengend Snippet: Figure 5. FUS, but Not TDP-43, HuR, SF1, or TIA-1, Shuttles from the Nucleus to the Cytoplasm after PARP-1 Activation in H2O2-Treated Cells (A) The relative distribution of PAR and five other RNA-binding proteins—SF1, TDP-43, TIA-1, FUS, and HuR—between the cytoplasm and nucleus of HeLa cells was measured at different times after H2O2 treatment (300 mM). The integrated fluorescence intensities were measured at the single-cell level by using cell profiler software and secondary antibody fluorescence. Statistical analysis of protein translocation was performed under the indicated conditions. Horizontal bars indicate mean; n > 244. Note that FUS translocation takes place after 15 min, which is not observed for TDP-43. (B) Upper left panel: anti-PAR fluorescence after H2O2 treatment (300 mM) for 10 min in the presence or absence of olabarib (10 mM). Upper right panels: sub- cellular FUS distribution after the indicated treatments. Lower panels: statistical analysis of FUS translocation under indicated conditions. H2O2 treatment: 300 mM, 90 min. Horizontal bars indicate mean. **p < 0.05, paired t test; n.s., non-significant; n > 278. Olaparib treatment or silencing PARP-1 inhibits the shuttling of FUS from the nucleus to the cytoplasm. (C) Subcellular distribution of TDP-43 in HeLa cells under the same conditions as in (B). Olaparib does not impair the nucleocytoplasmic shuttling of TDP-43. (D) Left panels: subcellular distribution of HuR in HeLa cells under the indicated conditions. Right panel: analysis of the nucleocytoplasmic shuttling of HuR. **p < 0.05, paired t test; n = 240. Actinomycin D, ActD: 5 mg/mL. Scale bar, 10 mm. H2O2 treatment prevents the translocation of HuR in response to ActD in HeLa cells in a PAR-dependent manner. n.s., not significant.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-FUS Novus Biologicals Cat#NBP2-52874 Rabbit polyclonal anti-FUS (used only in the Figure S2a) Novus Biologicals Cat#NB100-565 Rabbit polyclonal anti-PARP1 Santa Cruz Biotechnology Cat#sc-7150 Mouse polyclonal anti-HuR Abcam Cat#ab54987 Goat polyclonal anti-TIA-1 Santa Cruz Biotechnology Cat#sc-1751 Mouse monoclonal anti-TDP-43 Santa Cruz Biotechnology Cat#sc-376532 Rabbit polyclonal anti-PAR Trevigen Cat#4336-BPC-100 Mouse monoclonal anti- PARG Santa Cruz Biotechnology Cat#sc-398563 Mouse monoclonal anti-Alpha-Tubulin Abcam Cat#ab7291 Mouse monoclonal anti-SF1 Invitrogen Cat#MA5-27159 Goat polyclonal anti-Rabbit IgG (H+L) Thermo Fisher Scientific Cat#11012 Goat polyclonal anti-Mouse IgG1 Thermo Fisher Scientific Cat#21121 Goat anti-Mouse IgG (H+L) LI-COR Cat#926-32210 Bacterial and Virus Strains Escherichia coli BL21(DE3) EMD Millipore Cat#69450 Chemicals, Peptides, and Recombinant Proteins Actinomycin D (ActD) Thermo Fisher Scientific Cat#11805017 Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 SYBR Green II RNA Gel Stain Thermo Fisher Scientific Cat#S7564 Nb.BsmI, nicking endonuclease New England BioLabs Cat#R0706S HiScribe T7 High Yield RNA Synthesis Kit New England BioLabs Cat#E2040S PAR Polymer Trevigen Cat#4336-100-01 Olaparib (AZD2281, Ku-0059436) Apexbio Technology Cat#A4154 Ni-NTA Agarose QIAGEN Cat#30210 GelRed Biotium Cat #41003 Experimental Models: Cell Lines Human: HeLa ATCC Cat #CRM-CCL-2 Oligonucleotides FlexiTube GeneSolution for PARG QIAGEN Cat#GS8505 FlexiTube GeneSolution for PARP1 QIAGEN Cat#GS14 AllStars Negative Control siRNA QIAGEN Cat#1027281 Calf thymus DNA Sigma Cat#D4522 Recombinant DNA Vector pT7-FLAG-MAT-Tag-2 Sigma Cat#E5030 Vector pBR322 New England BioLabs Cat#N3033S pET-FUS This paper N/A pET-FUS-DLCD This paper N/A pET-FUS_1-374 This paper N/A pET-FUS_1-454 This paper N/A pET-MBP-FUS_FL_6E Monahan et al., 2017 Addgene #98652 pET-MBP-FUS_FL_12E Monahan et al., 2017 Addgene #98655 pET-Hur This paper N/A pET-G3BP1 This paper N/A (Continued on next page) e1 Cell Reports 27, 1809–1821.e1–e5, May 7, 2019

Techniques: Activation Assay, RNA Binding Assay, Software, Translocation Assay